Transfer membrane, nylon(FD0218)

Transfer membrane, nylon

Product Number: FD0218

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It is a neutral, enhanced Nylon membrane. Based on the material it provides a superior tensile strength and is extremely resistant against alcohol, acetone, and many other organic solvents.Application examples:Southern- and Northern blot, Dot blot, DNA fingerprinting, ELISA, colony- and plaque lifting, macro- and microarrays.Detection systems: radioactivity, chemoluminescence, fluorescence

Detail
Material	Nylon 6.6.
Thickness	150±20 µm
Version	roll
Application	Ideal for DNA/RNA transfer, particularly for colony- and plaque-lifting, as well as for all multiple rehybridisations.Especially recommended for Northern-blots.
Binding Capacity	500 µg/cm²
Immobilisation	UV, baking
Directions for use	In a buffer pH of 6.5,it is uncharged and by means of the surface-standing amino- and carboxy-groups has superior hydrophilic qualities. The charge of the membrane (ζ-potential) may be altered through the pH of the buffer - the membrane is positively charged at pH <6,5 and negatively charged at pH >6,5.Membrane of particulary high DNA-binding capacity. Available as roll (pore size 0,2 µm) for high-flexible usage, and as disks (pore size 0,45 µm) for colony- and plaque-lifting. May be steam sterilised.Covalent bondage of transferred nucleic acids is achieved by cross linking (baking for 15 min at 80 °C or UV-cross linking for 20 sec at 260 nm). Due to the excellent signal-to-noise ratio of the membrane, very sensitive detection (single copy-gene detection) and multiple rehybridisation is possible without loosing sensitivity.
Load	Positive
Binding capacity	500 µg/cm²

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